Chemical Constituents from Cratoxylum cochinchinense and their Cytotoxic Activity

Abstract

Six isolated compounds; Vismiaquinone A (CF1), γ-tocotrienol (CF2), δ- tocotrienol (CF3), 7-geranyloxy-1,3-dihydroxyxanthone (CF4), Cochinchinone G (CF5), Cochinchinone A (CF6) were isolated from ethylacetate extracts of Cratoxylum cochinchinense. The isolation of hexanes extract of twigs of C. cochinchinense to afforded six known compounds; Cochinchinone A (CT1), Dulcisxanthone F (CT2), ßmahostin (CT3), 7-geranyl-1,3,7-trihydroxy-4-(3,3-dimethylallyl)-xanthone (CT4), 1,3,7- trihydroxy-2,4-di-isopropylxanthone (CT5) และ Dulcisxanthone B (CT6). The structure of isolated compounds were elucidated by basic NMR spectroscopy (1H, 13C, COSY, HSQC, HMBC) and compared with previous literature reviews. All isolated compounds were tested for in vitro cytotoxic activity against five human cancer cell lines. The xanthone showed cytotoxicity more than other compounds and the polarity of compounds played important role in cytotoxic activity. Furthermore, 1,3,7-trihydroxy- 2,4-di-isopropylxanthone (CT5) showed the most cytotoxic activity against five human cancer cell lines; breast (BT474), lung (CHAGO), liver (Hep-G2), gastric (KATO- 3), and colon (SW-620) as 1.05, 0.46, 0.46, 0.63 and 3.11 μg/mL, respectively.