Application Supplementation of Immunostimulant in Dried Bio-Flocs on Survival Rate of Nile Tilapia infected with Streptococcus agalactiae, DMST 17129

Abstract

A research was aimed to employ dumped bio-floc sediment to mix β-glucan and nucleotide in Nile Tilapia cultivation system as immunostimulants. Afterwards, Nile Tilapia was fed with immunostimulants with commercial feed with an objective to assess it efficiency via examination of survival rate of Nile Tilapia infected with Streptococcus agalactiae, DMST 17129. The 1-inch body size of Nile Tilapia was infected with 10 cells/ml Streptococcus agalactiae, DMST 17129. After that, it was demonstrated that the fish was infected with 105cells/ml Streptococcus agalactiae, DMST 17129 and its efficacy against infection was probed. Experimental design was completely randomized design as four experiment groups consisted of 20 fishes/ replication for 10 experiment days (supplemented feed was fed 3 days prior to pathogen injection and 7 days after injection). Thereafter, with 4 individual treatments as follows: 1) fed with commercial feed only, 2) fed with feed and dried bio-flocs: 3) fed with commercial feed together with dried bio-floc and β-glucan and 4) fed with commercial feed with dried bio-floc and nucleotide. Data collection related to survival rate was recorded to evaluate immunostimulants efficiency and relative percent survival was determined and their mean difference was statistically assessed through Duncan Multiple Range Test (P < 0.05). Results revealed that average survival rate of individual treatments were 63.33+5.77, 3.33+5.77, 6.67+5.77 and 3.33+5.77 % respectively with statistically significant differences (P<0.05) as relative percent survival rates were 9.53+8.25, 95.24+8.25, 90.47+8.25 and 95.24+8.25 % respectively. As a consequence, Dried bio-flocs with a supplementation and without a supplementation of immunostimulants as β-glucan and nucleotide could well increase survival rate of Nile Tilapia infected with Streptococcus agalactiae, DMST 17129.