Localization of related enzyme and metabolic flux in oleaginous species

Abstract

Just as important is the high amount of MCFA and LC-PUFA produced by Thraustochytrium sp. B01, a potential source for large-scale oil production for biodiesel and DHA. Heterotrophic growth of thraustochytrid has potential in coproducing saturated fatty acid (SFA) or medium chain fatty acid (MCFA) for biodiesel and long-chain polyunsaturated fatty acids (LC-PUFA), namely arachidonic acid (ARA), eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA) for use in nutraceuticals. In this study, we proposed new Thailand thraustochytrid strains on the basis of 18S rRNA sequencing. The result showed a close phylogenic relationship and highest percentage of similarity to Thraustochytrids sp. strain. Physiological screening including initial pH, seawater concentration, glucose concentration and temperature was carry out for optimization of culture conditions of Thraustochytrid sp. B01 for biomass and total fatty acid production. The lipid productivity of 0.35 g/L/h was obtained by Thraustochytrium sp. B01 with glucose as carbon source in baffled shake flask cultivation indicating a potential source for large-scale oil production for biodiesel and DHA. The production of NADPH for fatty acid biosynthesis is dependent of malic enzyme, isocitrate dehydrogenase and glucose-6-phosphate dehydrogenase, respectively.