Antioxidant activities and cosmetic application of stem barks of Garcinia pedunculata

Abstract

In this research, the antioxidant efficacy of the crude extract from stem bark of Garcinia pedunculata were investigated. The crude extract was derived from methanol solvent extraction and extracted with ethyl acetate solvent. Then, the crude extract of ethyl acetate was separated by column chromatography technique using acetone and hexane solvent system to obtain 6 sub-fraction parts (FR1-FR6). The antioxidant activity of sub-fraction 1 (FR1) did not be showed because of its limiting solubility in antioxidant testing solvent. The sub-fractions 2 - 6 (FR2-FR6) were tested for antioxidant activity by DPPH technique. It was found that, the sub-fraction 2 did not show the antioxidant activity. The sub-fractions 3 - 6 exhibited the efficiency of 50 percent DPPH free radical inhibition (IC) in the concentration ranges of 327.59 - 10.59 ug / mL. The sub-extraction part 6 (FR6) displayed the most effective DPPH inhibition with the value of IC 5050 equal to 10.59  0.40 which was compared with the standard Trolox with IC equal to 5.08  0.68. This indicated that the polarity of the crude extracts in each sub-fractions influenced on DPPH free radical inhibition. Moreover, the antioxidant activity results of these sub-fractions using ABTS technique were consistent with the results from DPPH technique. These promising results should be able to apply for the development of cosmetics and spa.